CELL-VU Sperm Counting Chamber (MFR# DRM-600)
Directions For Use
Semen Testing- DRM 600 |
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A![]() B ![]() C ![]() D ![]() |
General chamber preparation for semen testing Mix the sample thoroughly just before testing. Pipette one drop (approximately 4 microliters) of specimen. Place the specimen at the extreme edge of one of the sampling areas (figure A). Two tests can be performed using one Cell-VU® slide. Gently lower the cover glass over the specimen so that the edge of the cover glass just covers the sample (figures B and C).
For undiluted sperm count all motile and non-motile sperm within 10 small boxes of this grid. Divide this number by 2. This result is the concentration of sperm in millions/ml.
Suggestions: For increased accuracy, count all sperm within the entire grid (100 boxes). Multiply the count by 50,000 to obtain the total concentration of sperm/ml. Sperm can be immobilized by placing a small amount of sample in an appropriate container and then immersing it in hot water for several minutes If diluted specimen is used, follow the instructions and multiply by the dilution factor. |